Fig 3.
JEV 2′-O MTase mutant is highly attenuated in mice. (A) Neuroinvasiveness tests for WT and E218A mutant virus in mice. Groups of 3-week-old BALB/c mice (n = 6) were i.p. injected with 4 × 107 PFU of WT and E218A viruses. The mortality was monitored for 15 days or till death. The significance between survival curves from each group was analyzed by Kaplan-Meier survival analysis with log-rank tests. (B and C) Viral loads in mouse sera (B) and brain tissue (C) were measured by plaque assay at the indicated time postinfection. Dotted lines represent limits of detection.