Fig 6.

Effects of back mutations in hsa-miR-296-5p target sites on replication and inhibitory effects of hsa-miR-296-5p on EV71-HeN-luc. (A) Schematic representation of pEV71-HeN-luc constructs with hsa-miR-296-5p target sites and their corresponding back mutations. (B) Sequencing results of each mutant EV71 virus at the two mutation sites. (C) Effects of hsa-miR-296-5p on infectivity of WT (HeN) and mutant (HeN-2115, HeN-2896, and HeN-MD) viruses. hsa-miR-296-5p-transfected RD cells were infected with each virus at an MOI of 5. Viral titers were determined at 12 h postinfection. Data are representative of at least two independent experiments, with each determination performed in triplicate (mean ± SD of fold change). **, P < 0.01.