Fig 6.
One million and 0.1 million of T cells transferred to nude mice were sufficient to protect from lethality in conjunction with 7-day treatment with ST-246. One day prior to infection with 104 PFU of IHD-J-Luc, nude mice received an adoptive transfer with 1.0 or 0.1 million of T cells (blue closed and open squares) or of CD4+ (red closed and open triangles) or CD8+ T cells (green closed and open diamonds) from normal BALB/c mice and starting 1 day postinfection, were all treated with ST-246 for 7 days. Control mice were sham transferred with PBS 1 day before infection and after infection received vehicle or ST-246 (black closed and open circles) for 7 days. Mice were observed for lethality (A) and were subjected to bioimaging until day 14 or 15 for all surviving mice and additionally on days 21 and 30 for the group of mice that were reconstituted with 0.1 million of T cells or T cell subsets to confirm complete clearance of IHD-J-Luc from all organs. Recorded fluxes were used to calculate mean fluxes ± the SD in the nasal cavity (B), lungs (C), spleen (D), and liver (E). PBS/vehicle, 3 mice per group; PBS/ST-246, Tc 106/ST-246, and Tc 105/ST-246, 4 mice per group. The experiment was performed twice with similar results.