Fig 12.

Attenuated susceptibility of Arabidopsis double lox1 α-dox-1 mutant to TSWV infection. (A) Arabidopsis lox1 α-dox-1 double mutant and WT plants were mock inoculated or inoculated with TSWV. Representative disease symptoms at 17 d.p.i. are displayed. (B) Leaf discs were excised and assayed for electrolyte leakage at 17 d.p.i. Asterisks indicate significant differences in TSWV-inoculated plants compared with control plants (Student's t test: *, P = 0.1; **, P = 0.05; ***, P = 0.01). Data represent the means ± standard errors of eight replicates, each consisting of six plants that received the same treatment. (C) Comparison of relative viral loads estimated by RT-PCR in WT and lox1 α-dox-1 mutant plants at 17 d.p.i. −RT, control without RT. The same RT reactions were used to amplify RPL3A gene transcripts as a control.