Fig 2.
NS1 is SUMOylated by both SUMO1 and SUMO2/3 in vivo. HEK293A cells cotransfected with (+) or without (−) the indicated combinations of mammalian expression plasmids, including T7T7NS1, the dicistronic expression constructs Dual S1/I/U and Dual S3/I/U, an expression construct for the SUMO-deconjugating enzyme SENP1, and an empty expression vector, were analyzed by SDS-PAGE and immunoblotting using anti-T7 MAb (A), anti-SUMO2 PAb (B), anti-SUMO1 MAb (C), and anti-GAPDH MAb (D). NS1SUMO, SUMOylated NS1; HMW, high molecular weight.