Fig 9.
NS1 SUMOylation affects the abundance of NS1 dimers and trimers. HEK293FT cells were cotransfected with (+) or without (−) the indicated mammalian expression constructs. At 24 h posttransfection, the cells were treated with either 1 mM disuccinimidyl glutarate (DSG) or vehicle only. Half an hour later, the cells were collected and analyzed by SDS-PAGE and immunoblotting using anti-T7 MAb (A and D), anti-Ubc9 MAb (B and E), and anti-GAPDH MAb (C and F). NS1SUMO, SUMOylated NS1. The positions of NS1 dimers and trimers are indicated and emphasized by diagonal arrows.