Fig 4.

Immunohistochemistry for ISG15 in brain and skeletal muscle. A rabbit polyclonal antibody against ovine ISG15 protein was stained with DAB chromogen (brown) on the brains (A, B, and C) and skeletal muscle (D, E, and F) from 45-dg control fetuses (A and D) and from infected fetuses at 14 dpi (45 dg) (B and E) and 28 dpi (63 dg) (C and F). Numerous cells in the brains and skeletal muscle of infected fetuses are positive for ISG15. No positive cells were observed in the tissues of control fetuses.