Fig 5.

Role of the AR2 region in NS5A transcriptional activity and HCV replication efficiency. The AR2 region of the NS5A transactivation domain of strongly (NS5A-v1) and weakly (NS5A-v5) transactivating NS5A variants were swapped (left panel). The effects of these exchanges on NS5A transactivation capacities and RNA replication were studied in the yeast one-hybrid system (black bars) and in the subgenomic replicon model (white bars), respectively. Error bars indicate SEM. The results are indicative of three independent experiments.