Fig 3.

The E2 Y18H mutation affects RRV replication in a cell-type-dependent manner. (A to F) C2C12 murine muscle cells (A and B), primary human synovial fibroblasts (C and D), or C6/36 mosquito cells (E and F) were inoculated with RRV-T48 or RRV-T48 E2 Y18H at an MOI of 0.01 (A, C, and E) or 5 (B, D, and F). At 0 hpi (input) and 1, 6, 12, 18, and 24 hpi, the amounts of infectious virus present in culture supernatants were quantified by plaque assays. Each data point represents the arithmetic mean ± standard deviation. Data were evaluated for statistically significant differences by two-way ANOVA followed by Bonferroni's test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. (G) C2C12 murine muscle cells were inoculated with RRV-T48 or RRV-T48 E2 Y18H at an MOI of 0.01 and incubated at 37°C or 30°C. At 0 hpi (input) and 1, 12, and 24 hpi, the amounts of infectious virus present in culture supernatants were quantified by plaque assays. Each data point represents the arithmetic mean ± standard deviation. Data were evaluated for statistically significant differences by two-way ANOVA followed by Bonferroni's test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.