Fig 5.

Disease symptoms induced by the cloned genomic DNAs of three genotypes of ToLDeV. The PA98-1-1 and PA98-2-1 variants came from samples of tomato leaf curl disease collected in 1998, whereas the PA10-3 strain came from a sample collected in 2010. From left to right are shown the empty vector (EV) control and symptoms induced by the PA98-1-1 and PA98-2-1 variants and PA10-3 strain in tomato (cultivar Glamour) (A) and Nicotiana benthamiana plants (B) at 50 and 25 days after agroinoculation, respectively. (C) Viral DNA levels in newly emerged leaves of infected tomato plants (cultivar Glamour) at 21 and 60 days postagroinoculation with the ToLDeV genotypes, as determined by DNA gel blot hybridization with a probe composed of a mixture of the cloned DNA components of these genotypes. The numbers 1 and 2 represent individual plants. M, the size marker (in kb). (D) Biomass of tomato plants determined 50 days after agroinoculation with the EV control, the PA98-1-1 and PA98-2-1 variants, and the PA10-3 strain. Biomass is presented as a percentage relative to the EV control. Bars with the same letters are not significantly different (P > 0.05) according to Tukey's test.