Replication competence and infectivity of ToLDeV mutants. (A) Results of replication assays in Nicotiana benthamiana leaf discs for ToLDeV wild-type (wt) and v1, c1, c4, and c5 mutants of the genomic DNAs of three genotypes of ToLDeV: the PA98-1-1 and PA98-2-1 variants and the PA10-3 strain. M, size marker (in kb). (B to G) Disease symptoms induced by c4 mutants of the three ToLDeV genotypes in tomato (cultivar Glamour) and N. benthamiana plants. From left to right, the empty vector control and symptoms induced by the c4 mutants and wild type in tomato plants for the PA98-1-1 variant (B), PA98-2-1 variant (C), and PA10-3 strain (D) and in N. benthamiana plants for the PA98-1-1 variant (E), PA98-2-1 variant (F), and PA10-3 strain (G).