Table 3.
Infectivity, partial host range, and symptomatology of three genotypes of ToLDeV from Perua
| Host plant | ToLDeV genotypeb | No. infected/no. inoculated (% infected)c | Symptom(s)d |
|---|---|---|---|
| Solanum lycopersicum cv. Glamour | PA98-1-1 | 49/50 (98) | dg, s, ulc, vp, vs |
| PA98-2-1 | 47/50 (94) | dg, s, ulc, vp, vs | |
| PA10-3 | 46/50 (92) | dg, ss, ulc, vp, vs | |
| Nicotiana benthamiana | PA98-1-1 | 28/28 (100) | s, dlc, vs |
| PA98-2-1 | 28/28 (100) | s, dlc, vs | |
| PA10-3 | 28/28 (100) | s, svs, ulc | |
| N. tabacum cv. Samsum | PA98-1-1 | 15/18 (83) | si |
| PA98-2-1 | 10/18 (56) | si | |
| PA10-3 | 11/19 (58) | si | |
| N. glutinosa | PA98-1-1 | 16/16 (100) | si |
| PA98-2-1 | 14/14 (100) | si | |
| PA10-3 | 13/14 (93) | si | |
| Datura stramonium | PA98-1-1 | 18/19 (95) | si |
| PA98-2-1 | 15/18 (83) | si | |
| PA10-3 | 10/19 (53) | si | |
| Chenopodium quinoa | PA98-1-1 | 0/13 (0) | ni |
| PA98-2-1 | 0/12 (0) | ni | |
| PA10-3 | 0/16 (0) | ni | |
| C. amaranticolor | PA98-1-1 | 0/13 (0) | ni |
| PA98-2-1 | 0/13 (0) | ni | |
| PA10-3 | 0/19 (0) | ni | |
| Cucurbita pepo cv. Small Sugar | PA98-1-1 | 0/16 (0) | ni |
| PA98-2-1 | 0/16 (0) | ni | |
| PA10-3 | 0/19 (0) | ni | |
| Phaseolus vulgaris cv. Topcrop | PA98-1-1 | 0/12 (0) | ni |
| PA98-2-1 | 0/12 (0) | ni | |
| PA10-3 | 0/17 (0) | ni |
Viruses were delivered by agroinoculation. Infectivity was determined 18 to 21 days postagroinoculation and based on symptom development (in tomato and N. benthamiana) and detection of viral DNA by PCR with the PAR1c496/ToLC-v1730 primer pair.
Genotypes included the PA98-1 and PA98-2 variants, represented by the PA98-1-1 and PA98-2-1 isolates, respectively, and the PA10 strain, represented by the PA10-3 isolate.
Results represent data from at least three independent experiments.
Symptom abbreviations: dg, distorted growth; dlc, downward leaf curling; ni, no infection (no symptoms and no viral DNA detected in newly emerged leaves); si, symptomless infection; s, stunting; ss, severe stunting; ulc, upward leaf curling; vp, vein purpling; vs, vein swelling; svs, severe vein swelling.