Table 2.
Scaffolding value of different paired-read library combinations
| No. of libraries* | Most efficient | Scaffold N50 | Least efficient | Scaffold N50 |
|---|---|---|---|---|
| 1 |
15 kb |
163,475 |
PE |
37,694 |
| 2 |
5 kb + 25 kb |
522,027 |
PE + 3 kb |
46,699 |
| 2 |
5 kb + 20 kb |
474,308 |
PE + 5 kb |
141,403 |
| 2 |
8 kb + 25 kb |
470,890 |
PE + 25 kb |
142,007 |
| 3 |
5 kb + 20 kb + 25 kb |
834,964 |
PE + 3 kb + 5 kb |
158,525 |
| 3 |
5 kb + 15 kb + 25 kb |
789,954 |
PE + 3 kb + 8 kb |
171,253 |
| 3 |
8 kb + 20 kb + 25 kb |
726,289 |
PE + 3 kb + 25 kb |
198,696 |
| 7 | ALL | 1,287,609 | N/A | N/A |
*All libraries were normalized to 8.5x physical genome coverage, limited by the amount of available data for the paired-end (PE) library.