Figure 11.

Partial dynamic electrophoretic fingerprint (DEF) of primary CD4⁺ T cells. 10⁷ purified CD4⁺ T cells (A) resting or (B) activated were suspended in 154 mM NaCl and titrated from pH 3.0 to 9.0 in increments of 0.5 pH units where electrophoretic mobility was measured. Activation was induced by treatment with phytohemagglutinin and interleukin-2 overnight. DEFs were generated from three titrations were made for each of three donors, with three measurements made at each titration point, which were averaged (n = 27 per titration point) and plotted using direct linear interpolation.