Figure 1.

LFA-1 MAb does not directly inhibit HIV replication via binding to virions. (A) A virus capture assay was used to determine whether LFA-1 MAb binds to HIV-1. Triplicate wells were coated with 5 μg/ml of LFA-1 MAb or PBS as a control. Undiluted virus stocks were then added to each well and the presence of bound virus was determined using a p24 ELISA. Results are expressed as the concentration of captured p24 divided by the concentration of p24 in the undiluted virus stock. (B) PHA-activated CD8+ cell depleted PBMC from 10 HIV-1 negative subjects were infected in triplicate with the same three HIV-1 isolates in the presence of increasing concentrations of LFA-1 MAb. The degree of HIV-1 replication was determined using a p24 ELISA on the supernatant at day 7. Each dot represents a single subject. The line represents the mean for each concentration of LFA-1 MAb tested. There was no significant difference in replication in the presence of LFA-1 MAb as compared to PBS only control for any of the viruses tested.