Figure 5.

Search for the identity of the anti-viral soluble factor. (A) Supernatants with anti-viral activity, obtained from culturing PBMC in the presence of LFA-1 MAb, were tested for MIP1-alpha, MIP1-beta, and RANTES using a bead based ELISA assay. Results are expressed as the concentration of each analyte in paired control and LFA-1 MAb treated supernatants. (B) Supernatants were then tested for the presence of 174 different analytes using a fluorescence based antibody array. Results from this semi-quantitative assay are expressed in relative light units. Epidermal Growth Factor (EGF) was the only analyte where the signal intensity differed significantly between LFA-1 MAb treated and control supernatants (p = 0.0313). (C) To confirm this finding, the concentration of EGF was then determined by standard quantitative ELISA. Unless noted, the difference between LFA-1 MAb and control treated supernatants were not significant.