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. 2013 Apr 27;14:285. doi: 10.1186/1471-2164-14-285

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Copyright © 2013 Neuhaus et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Up regulation of internalin and other in vivo-relevant genes upon acid shock. Transcriptional induction of selected genes transcriptionally induced after acid shock at 25°C (black columns) compared to acid shock at 37°C (grey columns) as deduced by microarray analysis. All genes have experimentally been demonstrated to play a role in listerial virulence (Additional file 2). Samples were taken 15, 30, 60 and 120 min after acid shock, and from acid-adapted cells. Two biological and two technical replicates were performed on microarray for each time point measured.