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. 2013 Apr 27;14:285. doi: 10.1186/1471-2164-14-285

Table 2.

List of genes identified in this study that are influenced by PrfA, σB, or VirR, are required for epithelial cell invasion or intracellular replication, are induced during intracellular replication, or are homologs of internalin

Locus
 Gene
 Functional annotation or prediction
 Comments from literature
 T
 min after acid shock
 Adaptation1
          15 30 60 120  
Induced after acid shock at both 25°C and 37°C
lmo0206
  
 Hypothetical protein
 PrfA-influenced, up regulated intracellularly [38]
 25°C
 1*
 2.0
 3.2
 3.1
 0.9
37°C
 1.4
 4.1
 2.4
 1*
 1*
lmo0223
 cysK
 CysK, highly similar to cysteine synthase
 PrfA-box upstream, cell wall proteome [47]
 25°C
 2.3
 6.2
 6.6
 4.1
 n.a.
37°C
 2.5
 3.9
 2.5
 2.9
 2.0
lmo0593
  
 Nitrate transporter
 Up regulated intracellularly [38]
 25°C
 0.6
 1*
 1.9
 2.2
 0.4
37°C
 6.2
 5.7
 6.6
 2.9
 1*
lmo0596
  
 Hypothetical protein
 Contains PrfA- and RpoS-boxes [48], σB-dependent [35]
 25°C
 0.8
 1.7
 2.0
 1.7
 0.5
37°C
 10.1
 5.9
 4.6
 3.3
 5.7
lmo0610
 inl
 Peptidoglycan bound protein (LPXTG motif)
 Internalin-homolog [49]
 25°C
 1.5
 3.7
 6.4
 5.7
 0.4
37°C
 9.1
 5.9
 5.4
 1*
 1*
lmo0669
  
 Oxidoreductase
 σB[50] and PrfA-regulated [48], required for intracellular survival [51]
 25°C
 1.8
 4.4
 8.3
 7.7
 0.7
37°C
 3.6
 5.7
 5.0
 3.7
 3.8
lmo0759
  
 Lactoylglutathione lyase
 Mutant is intracellularly attenuated [39]
 25°C
 2.6
 4.9
 8.2
 11.0
 0.8
37°C
 4.8
 4.9
 4.5
 2.9
 1*
lmo0783
  
 Mannose-specific PTS component IIB
 σB-dependent [52]
 25°C
 0.7
 1*
 2.2
 2.0
 0.8
lmo0880
  
 Cell wall associated protein precursor (LPXTG motif)
 Up regulated intracellularly [38], σB-regulated [53]
 25°C
 2.4
 6.9
 9.1
 8.8
 1*
37°C
 50.1
 18.8
 12.5
 23.9
 17.4
lmo1138
 clpP
 Proteolytic component of Clp protease
 Up regulated intracellularly [38]
 25°C
 n.a.
 1.8
 3.4
 4.5
 n.a.
37°C
 2.0
 4.5
 2.8
 1.4
 n.a.
lmo1293
 glpD
 Glycerol-3-phosphate dehydrogenase
 Up regulated intracellularly [38]
 25°C
 n.a.
 4.9
 7.7
 5.9
 n.a.
 
  
  
 Required for intracellular growth [39]
 37°C
 5.7
 4.4
 3.3
 5.3
 7.0
lmo1302
 lexA
 SOS response regulator
 Up regulated intracellularly [38]
 25°C
 1.8
 2.8
 3.5
 5.0
 1*
37°C
 2.0
 2.4
 3.5
 2.2
 1*
lmo1416
  
 Hypothetical protein
 Deletion renders L. monocytogenes bile sensitive [36]
 25°C
 1*
 1.7
 7.8
 11.5
 4.7
37°C
 1*
 8.9
 7.7
 9.4
 5.9
lmo1734
 gltS
 Glutamate synthase (large subunit)
 Intracellularly up regulated [39]
 25°C
 1.7
 2.0
 5.2
 1*
 1*
37°C
 1*
 6.9
 6.0
 1*
 2.4
lmo1991
 ilvA
 Threonine dehydratase
 Involved in intravacuolar survival [38], intracellularly up regulated [39]
 25°C
 1.7
 2.1
 2.9
 3.1
 0.9
37°C
 2.7
 3.3
 3.8
 2.9
 1*
lmo2085
  
 Peptidoglycan bound protein (LPXTG motif)
 Up regulated intracellularly [38], σB-dependent [53]
 25°C
 1.7
 6.3
 9.4
 7.3
 0.7
37°C
 6.1
 5.8
 3.9
 4.2
 1*
lmo2156
  
 Hypothetical protein
 VirR regulated [54]
 25°C
 8.3
 18.0
 11.1
 4.6
 2.0
37°C
 6.2
 9.9
 4.6
 4.6
 1.7
lmo2191
 spxA
 Hypothetical protein
 Up regulated intracellularly [38]
 25°C
 1*
 1.9
 3.6
 3.6
 0.8
37°C
 6.2
 4.7
 4.1
 4.1
 2.8
lmo2199
 ohrA
 Hypothetical protein
 Involved in hydroxyperoxide resistance [55], up regulated intracellularly [38]
 25°C
 1*
 1.7
 3.4
 7.4
 1.5
37°C
 1.5
 2.1
 3.8
 3.9
 1*
lmo2200
 ohrR
 Transcription regulator
 Involved in hydroxyperoxide resistance [55], up regulated intracellularly [38]
 25°C
 1*
 2.5
 5.2
 10.2
 2.0
37°C
 9.8
 11.2
 11.2
 14.0
 3.4
lmo2206
 clpB
 similar to endopeptidase Clp ATP-binding chain B
 chaperone, up regulated intracellularly [38,39]
 25°C
 2.9
 5.8
 5.8
 4.1
 n.a.
37°C
 7.2
 6.9
 3.5
 1.5
 n.a.
lmo2434
 gadD3 /gadD
 Glutamate decarboxylase
 Up regulated during acid stress [15] and intracellularly [39], σB-dependent [56]
 25°C
 1*
 5.7
 10.1
 11.1
 0.4
37°C
 1.8
 2.5
 2.4
 1*
 1.5
lmo2570
  
 Integral membrane protein
 PrfA-regulated [48]
 25°C
 0.7
 2.5
 5.8
 7.6
 0.8
37°C
 2.1
 4.1
 3.4
 2.7
 1*
lmo2571
  
 Nicotinamidase
 PrfA-regulated [48]
 25 °C
 0.5
 4.1
 3.4
 2.7
 1*
37°C
 12.6
 3.6
 8.2
 10.1
 1*
lmo2572
  
 Dihydrofolate reductase, chain A
 PrfA-regulated [48]
 25°C
 1*
 3.9
 8.6
 9.1
 1*
37°C
 5.5
 3.9
 n.a.
 3.7
 2.3
lmo2573
  
 Zinc-binding dehydrogenase
 PrfA-regulated [48]
 25 °C
 2.3
 6.8
 10.6
 10.0
 0.5
37°C
 14.3
 6.1
 6.2
 4.3
 6.5
lmo2695
  
 Dihydroxyaceton kinase
 Mutant attenuated in macrophages (Fuchs et al., unpublished data)
 25°C
 1*
 5.5
 8.2
 5.9
 0.8
37°C
 2.8
 2.6
 2.6
 2.8
 2.1
lmo2696
  
 Dihydroxyaceton kinase
 Mutant attenuated in macrophages (Fuchs et al., unpublished data)
 25°C
 n.a.
 5.3
 13.5
 18.6
 n.a.
37°C
 1.8
 5.5
 3.2
 2.3
 1.7
lmo2713
  
 Secreted protein with GW repeat
 Up regulated intracellularly [38], σB-regulated [48]
 25°C
 1.5
 3.0
 5.0
 3.9
 1*
37°C
 3.1
 2.5
 4.0
 2.8
 1.5
Induced after acid shock at 25°C, but not at 37°C
lmo1538
 glpK1
 Glycerol kinase
 Up regulated intracellularly [39]
 25°C
 4.3
 3.5
 2.5
 1.5
 4.6
Induced after acid shock at 37°C, but not at 25°C
lmo0232
 clpC
 Endopeptidase Clp, ATP-binding chain C
 Plays a role in adhesion and invasion, and modulates the expression of InlA, InlB and ActA [57]
 37°C
 2.7
 9.6
 6.0
 2.0
 1.8
lmo1439
 sod
 Superoxide dismutase
 Mutant attenuated in survival within macrophages [58]
 37°C
 1*
 1*
 2.5
 2.7
 2.0
lmo2250 arpJ ABC-transporter for arginine Mutant attenuated in intracellular growth [59] 37°C 1* 2.3 2.0 2.0 1*

See legend of Table  1.