Figure 4.

Aberrant tangential distribution of PV-interneurons in Disc1-L100P adult mice. (A) Four sampling ROIs immunolabeled with either CB or PV antibodies were outlined across the neocortex along the medial-lateral axis. The number of CB⁺ and PV⁺ cells were counted and expressed as a percentage of total cells in each ROI. (B) There was no significant difference in the tangential distribution of CB⁺ cells between WT and Disc1-L100P mice (n = 12–13 ROIs from 4 different mice per group). (C) PV-labeled cells were distributed differently depending on genotype. The Disc1-L100P mutants had a significantly higher proportion of PV⁺ cells located laterally (bin 1) but fewer PV⁺ cells in medial bin 4 when compared to WT (n = 22 ROIs from 4 different mice per group; two-way ANOVA, t-test, p < 0.05). Scale Bar, 500 μm. All data are shown as mean ± SEM; * p < 0.05 versus WT. CB, calbindin; PV, parvalbumin; ROI, region of interest.