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. 2013 Apr 27;6:31. doi: 10.1186/1756-8722-6-31

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Copyright © 2013 Falcon et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Time course of cord formation in ADSC and ECFC co-cultures grown in basal medium. (a) Images of endothelial cells stained with CD31 and SMA-positive pericytes in the ADSC/ECFC co-culture assay system either unstimulated (basal; top) or stimulated with 20 ng/mL VEGF (bottom) at 0, 1, 3, 5, and 7 days. (b) Graphs of a time course from 0–7 days showing total tube area of the cords (left) and its associated SMA index (right). (c) Continuous monitoring of the tube length per unit area of GFP labeled ECFCs in the ADSC/ECFC co-culture assay system in basal media plus with no stimulation (gray) or a concentration response of VEGF from 0 to 102 hours.