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. 2013 Apr 27;6:31. doi: 10.1186/1756-8722-6-31

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Copyright © 2013 Falcon et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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VEGF stimulated cords become insensitive to inhibitors of VEGF signaling in the ADSC/ECFC co-culture model. (a) Cords were established for 0, 1, 2, or 4 days, then treated with a concentration response of bevacizumab (Bev; left) or ramucirumab (Ram; right). Total tube areas for each were graphed. (b) Continuous monitoring of the effect of a concentration response of bevacizumab (Bev) on cord tube length beginning on day 0 (neoangiogenic mode; top) or after 4 days of establishment (established mode; bottom). (c) Continuous monitoring of the effect of a concentration response of ramucirumab (Ram) on cord tube length beginning on day 0 (neoangiogenic mode; top) or after 4 days of establishment (established mode; bottom).