Table 2.
Comparison of oligo design programs
| Program | Total oligos | Bad oligos | Good oligos | Specificity (%) | Coverage(%) | Time (s) | Speed (KB/s) |
|---|---|---|---|---|---|---|---|
| ArrayOligoSelector |
365,571 |
289,103 |
76,468 |
20.92 |
32.38 |
249,467 |
2.28 |
| OligoArray |
223,103 |
172,817 |
50,286 |
22.54 |
21.30 |
1,568,961 |
0.36 |
| OligoPicker |
269,971 |
54,715 |
215,256 |
79.73 |
91.16 |
98,343 |
5.77 |
| OligoWiz |
376,965 |
281,080 |
95,885 |
25.44 |
40.61 |
762,536 |
0.74 |
| PICKY |
166,126 |
10,697 |
155,429 |
93.56 |
65.83 |
4,745 |
119.64 |
| YODA |
225,332 |
4,190 |
221,142 |
98.14 |
93.66 |
1,577,385 |
0.36 |
| BOND | 236,111 | 0 | 236,111 | 100.00 | 100.00 | 862 | 658.59 |
The specificity represents the percentage of good oligos out of the total number. The maximum number of genes that have good oligos was estimated by our evaluation program at 236,122 oligos. The coverage represents the percentage the number of good oligos represents out of this estimated maximum.