Skip to main content
. 2013 May 8;8(5):e63012. doi: 10.1371/journal.pone.0063012

Figure 1. Oxyclozanide binds S100A9 protein.

Figure 1

A: General formulae of synthesized salicylic and benzoic amides. B: Structure of Oxyclozanide (OX). C-D: Inhibition of S100A9 binding to immobilized RAGE (C) or TLR4 (D) by OX. 50 nM S100A9 was injected over RAGE or TLR4±0.4–200 µM OX in the presence of 1 mM CaCl2, 20 µM ZnCl2 and 1% DMSO. Binding was expressed as % inhibition of S100A9 response without OX and IC50 calculated after curve fit to a sigmoidal dose-response model. IC50 values of ∼1.3 and 1.6 µM were obtained for OX inhibition of S100A9 binding to RAGE and TLR4. E: Effect of Zn++ on direct binding of OX to amine coupled S100A9. 3.125–100 µM OX was injected (2 min at 30 µL/min) over S100A9 (density ∼2.6 kRU) in HBS-P with 1 mM Ca++ ±20 µM Zn++. Responses at late association phase are plotted versus OX concentration and curves fit to a 1∶1 model. Zn++ specific binding was obtained by subtraction of responses with Ca++ alone.