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. 2013 May 8;8(5):e63587. doi: 10.1371/journal.pone.0063587

Figure 2. DEX pre-treatment and NDV infection cooperatively increased IL-10 mRNA and protein expression in DCs.

Figure 2

DCs were pre-treated with DEX (A: 10−6 M, B: 10−6, 10−7 or 10−8 M) or CORT (C: 10−6 M) for 30 min, and were infected with NDV (MOI = 10) for 6 hours. Time-course of IL-10 mRNA (A, left panel) and protein (A, right panel) expression, and the effect of increasing concentrations of DEX on these parameters (B, left and right panel, respectively) are shown. Time-courses of IL-10 mRNA (left panel) and protein (right panel) expression obtained in the presence of CORT pre-treatment are also shown in panel C. Relative IL-10 mRNA expression (fold changes) was calculated by comparing to the baseline (the conditions at time “0″ or those obtained in the absence of DEX/CORT pre-treatment and NDV infection). Bars represent means and standard errors of fold changes of the IL-10 mRNA expression and protein concentrations in the culture media obtained from three independent experiments. *: p<0.05, **: p<0.01, ***: p<0.001, compared to the conditions obtained in the presence of DEX or CORT treatment alone. Ctl: control.