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. 2013 May 8;8(5):e63587. doi: 10.1371/journal.pone.0063587

Figure 4. GR mediated DEX pre-treatment- and NDV infection-induced cooperative IL-10 mRNA expression in DCs.

Figure 4

A. RU486 suppressed DEX-induced increase of IL-10 mRNA and protein expression in DCs. DCs were pre-treated with RU486 (10−5 M) and/or DEX (10−6 M) for 30 min, and were infected with NDV (MOI = 10) for 6 hours. IL-10 mRNA and protein expression are shown. Bars represent means and standard errors of fold changes of the IL-10 mRNA expression and protein concentrations in culture media obtained from three independent experiments. **: p<0.01, compared to the 2 conditions indicated. B & C. DEX pre-treatment and NDV infection did not change GR mRNA and protein expression in DCs. DCs were pre-treated with DEX (10−6 M) for 30 min, and were infected with NDV (MOI = 10) for 6 hours. Bars in panel A and B represent means and standard errors of fold changes of the mRNA expression obtained in three independent experiments. Whole cell extracts obtained from DCs were run on SDS-PAGE gels, and GR and control β-actin were visualized with their specific antibodies in Western blots in panel C.