Figure 8. NDV increases DEX-induced expression of several glucocorticoid-responsive genes through phosphorylation of GR.

A. NDV increased DEX-induced mRNA expression of 3 well-known glucocorticoid-responsive genes in DCs. DCs were pre-treated with DEX (10⁻⁶ M) for 30 min, and were infected with NDV (MOI = 10) for 6 hours. mRNA levels of DUSP1, GILZ and PER1 were measured in the real-time qPCR. Bars represent means and standard errors of fold changes of their mRNA expression obtained in three independent experiments. **: p<0.01, compared the 2 conditions indicated. B: ERK2 increased DEX-induced transcriptional activity of the GILZ promoter in the presence of wild type human GR in HCT116 cells, while it lost the effect in the presence of the mutant GR harboring S203A replacement. HCT116 cells were transfected with ERK2 D319N-expressing plasmid in the presence of wild type human GR or its mutant with S203A replacement, together with the pGILZ-luc reporter and the pGL4.73[hRluc/SV40] control plasmid. Cells were subsequently treated with DEX (10⁻⁶ M) for 24 hours. Bars represent means and standard errors of the firefly luciferase activity corrected for renilla luciferase activity. **: p<0.01, n.s.: not significant, compared the 2 conditions indicated.