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. 2013 May 8;8(5):e63731. doi: 10.1371/journal.pone.0063731

Figure 5. Huaier regulates proteins related to cell proliferation, apoptosis and cell adhesion or invasion in ovarian cancer cells, as detected by the reverse phase protein array (RPPA) assay.

Figure 5

(A) Hierarchical cluster analysis of four samples (control, Huaier-treated for 72 hours, starvation+EGF stimulation, starvation +EGF stimulation+Huaier for 60 hours) in SKOV3 and SKOV3.ip1 cells according to the expression of 16 signaling molecules. Cluster color key: red – up-regulated; green – down-regulated; black – unchanged. (B and C): Fold changes in the expression or phosphorylation of various proteins after Huaier treatment and addition of EGF were analyzed using the RPPA assay in the SKOV3 (B) and SKOV3.ip1 (C) cells. The relative values of the control group and the starvation+EGF stimulation groups were set as 1.0. *: fold change>30%.