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. 2013 May 8;8(5):e63731. doi: 10.1371/journal.pone.0063731

Figure 9. Huaier could not inhibit β-catenin in SKOV3, SKOV3.ip1 and Hey cells after specific GSK3β siRNA transfection.

Figure 9

Cells were transfected with non-specific siRNA (lane 1, 5 and 9) and with GSK3β specific siRNA only (lane 2,6 and 10). Cells were transfected with non-specific siRNA (lane 3,7and 11) or GSK3β specific siRNA (lane 4, 8 and 12) for 24 hours and then treated with 5 mg/ml Huaier for additional 24 hours. Western blot results show GSK3β, phosphor-GSK3β pS9 and β-catenin expression. The basal level for the non-specific siRNA groups was assigned a value of 1.