Table 2.
The amino acid substitutions in the HA2 and character of Stachyflin-resistant (R) virus clones
| Virus | EC50 (μM) | Amino acid position in HA2a | ⊿pHc | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| |
|
|
37 |
51 |
85 |
91 |
98 |
107 |
110 |
|
| WSN |
Wild type |
0.02 |
D |
K |
D |
I |
L |
T |
F |
0.0 |
| |
R1 |
>6.50 |
N |
-b |
- |
- |
- |
- |
- |
0.3 |
| |
R2 |
>6.50 |
- |
R |
- |
- |
- |
- |
- |
−0.3 |
| |
R3 |
>6.50 |
- |
- |
H |
- |
- |
- |
- |
0.0 |
| |
R4 |
>6.50 |
- |
- |
- |
- |
- |
I |
- |
0.2 |
| |
R5 |
>6.50 |
- |
- |
- |
- |
V |
- |
- |
−0.2 |
| |
R6 |
>6.50 |
- |
- |
- |
F |
- |
- |
- |
0.0 |
| PR8 |
Wild type |
0.49 |
D |
K |
D |
I |
L |
T |
F |
N.D.d |
| |
R1 |
>6.50 |
- |
- |
- |
- |
S |
- |
- |
N.D. |
| |
R2 |
>6.50 |
- |
- |
- |
- |
- |
- |
S |
N.D |
| Ibaraki |
Wild type |
0.17 |
D |
K |
D |
I |
L |
T |
F |
N.D |
| |
R1 |
>6.50 |
- |
R |
- |
- |
- |
- |
- |
N.D. |
| Taiwan |
Wild type |
0.44 |
D |
K |
D |
I |
L |
T |
F |
N.D. |
| R1 | >6.50 | - | R | - | - | - | - | - | N.D. | |
a H3 subtype numbering.
b Dash (-) means the same amino acid as the wild type virus.
c The pH at which 50% hemolysis of the wild type virus is 6.0. The values indicate the difference of the pH at which 50% hemolysis between the Stachyflin-resistant virus clones and wild type virus.
d Not determined.