Figure 3. Binding curves from matched plasma samples with and without the addition of saturating (1 µM) recombinant FVIII-C2.

(A–F) Black arrows pointing downwards indicate injection of CA treated plasma and red arrows indicate injections of anti-huIgG₁, anti-huIgG₂, anti-huIgG₃ and anti-huIgG₄. Injection of FVIII and its capture on mAb GMA-8004 (0–700 s) are not shown. (G) The Biosensor assay format is shown schematically.