Table 1.
The frequency, fundamental lifetime, overtone lifetime, and node slope for the symmetric stretch of CpLx in either solvent or after reaction with the specified mutant.
| ω/cm−1 (±0.5) | T01/ps | T12/ps | Node Slope/ωpump÷ωprobe | ||
|---|---|---|---|---|---|
| DMSO | 1C | 2014.0 | 5.55 ± 0.05 | 6.14 ± 0.04 | 2.50 ± 0.03 |
| 3C | 2015.6 | 10.1 ± 0.2 | 9.97 ± 0.13 | 3.40 ± 0.23 | |
| 4C | 2014.2 | 7.91 ± 0.10 | 8.35 ± 0.08 | 4.37 ± 0.09 | |
| CH2Cl2/CCl4 | 1C | 2024.1 | 18.1 ± 0.4 | 16.1 ± 0.4 | 1.98 ± 0.07 |
| 3C | 2024.5 | 19.0 ± 0.6 | 18.9 ± 0.5 | 1.95 ± 0.10 | |
| 4C | 2022.4 | 14.2 ± 0.3 | 14.2 ± 0.2 | 5.13 ± 0.11 | |
| Ubiquitin K6C | 1C | 2022.2 | 9.13 ± 0.17 | 8.47 ± 0.19 | 1.63 ± 0.29 |
| 3C | 2019.4 | 12.8 ± 0.2 | 12.4 ± 0.1 | 1.77 ± 0.04 | |
| 4C | 2015.4 | 5.75 ± 0.12 | 4.67 ± 0.11 | 1.56 ± 0.42 | |
| Ubiquitin K63C | 1C | 2020.8 | 6.97 ± 0.13 | 5.80 ± 0.10 | 1.86 ± 0.21 |
| 3C | 2017.0 | 6.56 ± 0.19 | 5.67 ± 0.10 | 1.73 ± 0.19 | |
| 4C | 2020.4 | 19.7 ± 1.1 | 14.0 ± 0.5 | 1.86 ± 0.10 | |
| α-synuclein V71C buffer (4C Label) | 2020.7 | 11.1 ± 0.7 | 6.74 ± 1.65 | 3.75 ± 0.21 | |
| α-synuclein V71C vesicles (4C Label) | 2019.2 | 14.2 ± 3.1 | 9.09 ± 2.11 | 3.78 ± 0.11 | |