Table 1. Primers used in this study.

Primer	Name	Aligns to BUNS(+) nts	Sequence 5′–>3′
ivk01	S-Fw mut	1–46*	AGTAGTGTACTCCACCTAAAACTTATGCTATTGTTGAAAATCGCTG
ivk02	S-Rv mut	936–912*	CAGTTCTCGAGCATCAAATACCATTAACAATATAATGTTG
ivk20	NSs-start Fw	104–126*	GGGAAACGTCTCaCATGATGTCGCTGCTAACACCAG
ivk55	3′UTR Rv	892–846*	TCGAGTGTCCCCAACCACCCACCCAAGCAGCTGTTATTTTGTGGGTTGAAAC
ivk69	4KR- Fw	211–278	CTACATTAAgGGACGCGAGATTAAgACTAGTCTCGCAAAgAGAAGTGAATGGGAgGTTACACTTAACC
ivk70	4KR- Rv	273–203	AGTGTAACcTCCCATTCACTTCTcTTTGCGAGACTAGTcTTAATCTCGCGTCCcTTAATGTAGAAGATTCG
ivk86	K1-F	211–237	CTACATTAAaGGACGCGAGATTAAGAC
ivk87	K1-R	237–211	GTCTTAATCTCGCGTCCtTTAATGTAG
ivk88	K2-F	220–246	GGGACGCGAGATTAAaACTAGTCTCGC
ivk89	K2-R	246–220	GCGAGACTAGTtTTAATCTCGCGTCCC
ivk90	K3-F	235–263	GACTAGTCTCGCAAAaAGAAGTGAATGGG
ivk91	K3-R	263–235	CCCATTCACTTCTtTTTGCGAGACTAGTC
ivk92	K4-F	250–278	GAGAAGTGAATGGGAaGTTACACTTAACC
ivk93	K4-R	278–250	GGTTAAGTGTAACtTCCCATTCACTTCTC

^*primers with internal mismatch or 5′ tail that did not interfere with PCR amplification.

Mutations introduced by these primers are indicated in bold and lower case.