Skip to main content
. 2013 May 8;8(5):e62631. doi: 10.1371/journal.pone.0062631

Figure 2.

Figure 2

(a) Growth comparison of QM 9414 to the Δ3405 strain on minimal media supplemented with D-glucose, lactose and D-galactose. Pictures were taken on day 4. (b-g) Comparison of strains QM 9414 (grey), Δ3405 (white) and ptef::3405 (black) pregrown in minimal medium supplemented with 1% glycerol and,after washing of the mycelium extensivly with sterile water, transferred to minimal medium containing 1% lactose. (b) Accumulation of cel6a, cel7a and xyr1 transcripts measured by qPCR. Samples were taken 5, 8, 16 and 24 h after replacement to 1% lactose. The expression is given in relation to tef1 gene expression, where the tef1 expression value equals one. Mean values ± SD of three independent experiments are shown. (c) Lactose concentration in the medium during growth of QM 9414 (filled diamonds), Δ3405 (empty circles) and ptef::3405 (empty squares) on 1% lactose. (d) Biomass formation of strains QM 9414 (filled diamonds), Δ3405 (empty circles) and ptef::3405 (empty squares) on 1% lactose. Mean values ± SD of three independent experiments are given. (e) SDS-PAGE analysis of culture filtrates from QM 9414, Δ3405 and ptef::3405. Samples taken at 16 and 24 h after replacement to lactose. (f) ß-galactosidase activity determined with o-nitrophenyl-ß-D-galactopyranoside as the substrate. Error bars indicate the standard deviation of three independent experiments.