Figure 3. Diurnally synchronized syntrophic interaction with H.salinarum increases productivity of D. salina.

(A) Intra- and (B) extra-cellular glycerol concentrations in D. salina culture individually (blue) or with H. salinarum (red) over several day: night cycles, dash lines represent +/− standard deviation. (C) Radiolabel incorporation and tracing shows daytime uptake and nighttime release of ¹⁴C by D. salina. Uptake of ¹⁴C by D. salina at night is enhanced two-fold in co-cultures relative to pure cultures indicating nighttime assimilation of ¹⁴C in presence of H. salinarum. (D) Simultaneous tracing of C within H. salinarum cells demonstrates uptake and processing of ¹⁴C in sync with the diurnal cycle.