Figure 4. S1P stimulates redistribution of coronin 1B and cortactin to lamellipodia in human lung endothelial cells.

HPAECs grown on slide chambers (∼90% confluence) were stimulated with 1 µM S1P for different time interval (2, 5, 15, 30 and 60 min) as indicated. Redistribution of Coronin 1B (B) and Cortactin (C) was visualized by immunocytochemistry and quantified by ImageJ software as described in Materials and Methods. Shown are representative immunofluorescence images from several independent experiments.