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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Prog Retin Eye Res. 2013 Feb 5;35:18–43. doi: 10.1016/j.preteyeres.2013.01.004

Figure 5.

Figure 5

Targeted proteomics analysis of experimental rat glaucoma. As an effort to explore specific protein interactions in an experimental rat model of glaucoma, 14-3-3-containing retinal protein complexes were eluted using co-immunoprecipitation and recombinant protein-based affinity pull-down for subsequent analysis by mass spectrometry. Based on the proteomics data and in vivo treatment experiments in rats, 14-3-3 proteins were found to control the subcellular localization of and function of Bad in a phosphorylation-dependent manner, and thereby constitute an important regulatory pathway of RGC death signaling during glaucomatous neurodegeneration. This flow diagram has recently been published (Yang et al., 2008).