Skip to main content
. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Ann Hematol. 2013 Feb 1;92(6):777–787. doi: 10.1007/s00277-013-1684-6

Figure 5. ABC DLBCL cells lines were more sensitive to HhAntag than GC DLBCL cells. HhAntag induced a concentration-dependent decrease of the anti-apoptotic proteins BCL2 and BCL-W in ABC DLBCL cells.

Figure 5

Annexin V and PI labeling assays showed that the beneficial effect of adding fixed IC20 doses of ABT-737 to increasing concentration of HhAntag on the percentage of apoptosis was less pronounced in OCI-LY10 and OCI-LY3 cells than that seen in GC DLBCL cells (A). In contrast to those GC DLBCL cell lines with the t(14;18), treatment with increasing concentrations of HhAntag resulted in decrease of the expression levels of BCL2 and BCL-W in ABC DLBCL cell lines. In contrast to the GC DLBCL cell lines, OCI-LY3 and OCI-LY10 show the shortest form of BIM (BIM S). Three major isoforms of BIM are generated by alternative splicing: BIM EL, BIM L and BIM S [28].