Table 1.

Peak Assignments for normal Raman, SERS and TERS spectra

Raman	SERS			TERS			Details
SAa	SAa	SA-Bb	Biotin	50nm b-GNPc	80nm b-GNPc	50nm sa-GNPd	Assignment	Refs
				473	476	469	Phe	52
490	503	503	506		489		Tyr	52
538	539	540					Trp, Val	57
566	567	567		580	565		Trp	57
619	624	624			624	620	Phe	57
				661	667	663	ν-C-S	49
704	704	706					Trp(W18)	49
			715				Biotin
754				742	740		Trp(W18)	57
	813	812	813	817		813	Biotin/Streptavidin	58
827							Tyr(Y1)	57
849		854	860				Tyr(Y1)	57
876	883	887	885				Trp(W17)	57
925	941	942	934			932	Trp(W25),Glu,Asp	57
959	971	967			965	973	Trp, Val	57
	996	999	992	993	993		Biotin/Phe
1007	1014	1010	1007		1010		Trp(W16),Phe,Ala	57
		1048	1051	1050	1052		Biotin	59
1075	1077	1077				1072	Glu,Thr	57
			1082				ν-C-C	46
1095				1103			Ala,Lys	57
1123	1125	1126	1130	1133	1125	1124	ν-C-N,Trp(W13),Val,Glu	57
1154					1149		ν-C-N, Val, Lys	57
1175	1178	1176	1176		1171	1173	Phe,Tyr,Val	57
1204					1203		Streptavidin
	1221	1219				1218	Tyr(Y7a)	46
1235	1237	1238					AmideIII	57
			1242			1247	Ureido ring	46
1257	1274	1273	1274	1272	1272		Biotin, AmideIII	57,60
				1293	1304	1298	ω-CH2	46
1322	1310	1316	1317		1312		Ser(γ-CH2)	57
1332				1335			Trp(W7)	57
1356					1351	1350	Trp(W7)	57
		1372	1376				ω-CH2	46
1384	1380					1392	ν-COO-, ν-Cα-H	49
1411	1411	1411	1408				Biotin/Streptavidin
1448	1453	1453	1448				σ-CH2, CH3	57
			1461				ν-CH2 ring	46
	1482	1476		1473	1486	1485	Streptavidin
		1492	1496		1493		Biotin	60
		1504	1509		1504	1502	NA
1547	1560	1560	1561			1562	Trp(W3)	57
			1586	1580	1582		ν-C-N	46
	1591	1591				1592	Trp(W2)	57
		1639	1639				ν−C=O	46

^aSA - streptavidin;

^bSA-B - streptavidin-biotin complex;

^cb-GNP - biotinylated GNP;

^dsa-GNP - streptavidin-GNP