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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Eur J Neurosci. 2013 Feb 7;37(9):1383–1393. doi: 10.1111/ejn.12144

Figure 9. 20µM forskolin (FSK) markedly suppresses GABA-evoked currents (IGABA) recorded in wild type, dnc1 and rut1 pupal mushroom body (MB) neurons.

Figure 9

Cultured pupal MB neurons were prepared from wild type, dnc1 and rut1. MB neurons were identified by GFP expression under the control of a MB-specific driver c309-Gal4. GABA-evoked currents (IGABA) were suppressed in neurons derived from all three strains. Suppression for wild type, dnc1 and rut1 was 97.3, 92.0 and 92.9%, respectively. Although this suppression was slightly higher in wild type, there was no statistical difference between the three strains. In contrast, GABA-evoked currents (IGABA) from wild type embryonic MB neurons showed reduced sensitivity to FSK as it suppressed only 80.7%. Inset IGABA recorded from a wild type pupal MB neurons before and after 20µM FSK perfusion. Students t-test, **P< 0.01. Number of replicates: Wt embryonic (n=7) and pupal (n=4), dnc1 (n=4), rut1 (n=6).