Figure 2.

Long-term fluoxetine treatment does not affect distal dendritic spine morphology of CA1 pyramidal neurons. (A) Left: Golgi stained CA1 pyramidal neuron indicating the stratum lacunosum moleculare (s.l.m.) where the morphological analysis was performed. ^#At this level can be observed granule neuron dendrites coming from dentate gyrus. Scale bar: 100 μm. Right: representative dendritic segments of saline and fluoxetine-treated rats under larger amplification. Scale bar: 10 μm. (B) The number of spines and the morphology of spines were analyzed and plotted as spine density and % of spine type, respectively. Filopodia/thin (T), stubby (S), and mushroom/branched (M) shape categories are shown. Results are presented as mean ± SEM and were determined from 10 to 11 cells per condition, obtained from five saline (total spines, 979)- and six fluoxetine (total spines, 942)-treated rats. Data were not statistically significant after a Mann–Whitney U-test analysis.