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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Shock. 2013 May;39(5):447–452. doi: 10.1097/SHK.0b013e31828f9c92

Figure 3.

Figure 3

CD11c+ cells are a source of IL-23(p19) during acute lung injury. A, CD11c-diphtheria toxin receptor transgenic mice (CD11c-DTR Tg) and C57BL/6 mice received diphtheria toxin (4 ng/g body weight i.t. together with 25 ng/g body weight i.p.) 24 h before LPS-ALI. Splenocytes were harvested 8 h after LPS-ALI and stained for CD45 and CD11c by flow cytometry. B, Cells in BALF from C57BL/6 and CD11c-DTR Tg mice were stained for CD45 and CD11c after LPS-ALI in mice from the same experiment as described above. C, Detection of IL-23(p19) by ELISA in BALF 8 h after LPS-ALI with depletion of CD11c+ cells in CD11c-DTR Tg (n=4) mice as compared to C57BL/6 mice (n=8) as controls. Both groups had received treatments with diphtheria toxin as described above. D, Comparison of IL-23(p19) concentrations in BAL fluids 8 h after LPS-ALI in Rag-1−/− mice (n=5, deficient in T and B cells) and C57BL/6 mice (n=7).* P < 0.05, n.s. indicates not significant.