Figures 1.
Effects of midazolam on phorbol-12-myristate-13-acetate (PMA)-induced activation and expression of MMP-9 in chondrosarcoma SW1353 cell lines. Cell-free conditioned medium and supernatants were assayed for MMP-9 activation and expression by ge la tin zymography (A) and western blotting (B), respecti vely. Lane 1 is the culture medium of HT1080 cells in which the constitutive proMMP-2 and proMMP- 9 were expressed (A). SW1353 cells (2 × 106 cells/ml in 6-well plates) were treated with various concentrations of midazolam (5, 10, and 20 μM) or vehicle (DMSO) for 15 min before treatment with PMA (10 ng/ml) for 24 h. α-tubulin is used as an internal control (B)
Data are shown as the mean (SEM of three independent experiments). ###p < 0.001 compared with the untreated group, *p < 0.05 **p < 0.01, and ***p < 0.001, compared with the vehicle group