Figures 2.
Effects of midazolam on phorbol-12- myristate-13-acetate (PMA)-induced expression of MMP-1 (A) and MMP-13 (B) in chondrosarcoma SW1353 cell lines. Cell lysates were obtained and analyzed for MMP-1 and MMP-13 protein expressions by Western blotting. SW1353 cells (2 × 106 cells/ml in 6-well plates) were treated with various concentrations of midazolam (5 μM, 10 μM, and 20 μM) or vehicle (DMSO) for 15 min before treatment with PMA (10 ng/ml) for 24 h. α-Tubulin is used as an internal control
Data are shown as the mean (SEM of three independent experiments). ###p < 0.001 compared with the untreated group, *p < 0.05, **p < 0.01, and ***p < 0.001, compared with the vehicle group