Figures 4.
Effects of midazolam on phorbol-12- myristate-13-acetate (PMA)-induced expression of pERK1/2 (A) and p38 (B) in chondrosarcoma SW1353 cell lines. Cell lysates were obtained and analyzed for pERK1/2 and p-p38 protein expression by Western blotting. SW1353 cells (2 × 106 cells/ml in 6-well plates) were treated with various concentrations of midazolam (5 μM, 10 μM, and 20 μM) or vehicle (DMSO) for 15 min before treatment with PMA (10 ng/ml) for 24 h. α-Tubulin is used as an internal control
Data are shown as the mean (SEM) of three independent experiments. ###p < 0.001 compared with the untreated group, *p < 0.05, **p < 0.01 and ***p < 0.001, compared with the vehicle group