Figure 1.

Construction and identification of adenovirus Ad-RAR-β. A – Identification of recombinant pAd-RAR-β plasmid by PCR amplification and enzyme digestion. 1 – λ-HinD III DNA marker, 2 – full length of RAR-β PCR amplified from pAd-RAR-β, 3 – pAd-RAR-β digested by Pac I enzyme, 4 – pAdEasy-1 bone vector digested by Pac I enzyme as negative control. B – Package of Ad-RAR-β in HEK293 cells. After digestion by Pac I enzyme and purification, pAd-RAR-β was liposome transfected into HEK293 cells. a – pAd-RAR-β transfected HEK293 cells at 1d. b – Cloudiness amplification of adenovirus Ad-RAR-β was observed in HEK293 cells at 10d pAd-RAR-β transfection. C – Infected efficacy of adenovirus Ad-RAR-β in MSCs. a – Uninfected MSCs, b – Ad-null infected MSCs, c – Ad-RAR-β infected MSCs (Scale bar = 200 µm)