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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Nat Med. 2007 Feb 4;13(2):189–197. doi: 10.1038/nm1545

Figure 3. Aldosterone increases endothelial cell oxidant stress and decreases bioavailable NO•.

Figure 3

(a) Reactive oxygen species accumulation was measured by 6-carboxy-2′-7′ dichlorodihydrofluorescein diacetate (DCF) fluorescence in BAEC treated with aldosterone (ALDO) or vehicle (V) for 24 h and reported as arbitrary units (a.u.) ( * P < 0.01 vs. V, n = 8). (b) The corresponding reduced glutathione (GSH)/oxidized glutathione (GSSG) ratio was measured (*P < 0.05 vs. V, n = 6). (c) eNos activity was determined in intact endothelial cells in the absence of exogenous cofactors (*P < 0.05 vs. V, **P < 0.001 vs. V, n = 6). (d) eNos protein levels were examined; dimer-monomer levels were determined by low temperature gel electrophoresis, and (e) levels of bioactive NO• were determined by measuring cGMP in cells stimulated with bradykinin (5 μmol/L) (*P < 0.01 vs. V, **P < 0.005 vs. V, n = 8). Representative blots are shown. Data represent mean ± SEM.