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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Nat Med. 2007 Feb 4;13(2):189–197. doi: 10.1038/nm1545

Figure 4. Aldosterone and G6pd activity in vivo.

Figure 4

C3H wild-type mice were infused with aldosterone (50 μg/kg/day) (ALDO) or vehicle (V) via Alzet minipump for 14 d and (a) systolic blood pressure (SBP) was measured by tail cuff (*P < 0.001 vs. V; n = 40 per treatment group). After 14 d of aldosterone infusion, aortas were harvested and (b) G6pd expression was examined by immunohistochemistry; and (c) G6pd activity and (d) Nadph levels were measured in tissue homogenates (*P < 0.001 vs. V, n = 12). (e) Aorta reactive oxygen species formation was examined by dihydroethidine fluorescence and (f) lucigenin (5 μmol/L) chemiluminescence (*P < 0.001 vs. V, n = 12). (g) Levels of bioavailable NO• were assessed by cGMP levels (*P < 0.01 vs. V, n = 12). (h) eNos expression was examined in the aorta by immunofluorescence. Representative sections are shown; all sections magnified 200X. Data represent mean ± SEM.