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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Nat Commun. 2012 Mar 20;3:753. doi: 10.1038/ncomms1724

Figure 1. sigI expression and validation of M.tb. ΔsigI.

Figure 1

(a) Transcript copy numbers of sigI (closed circle) and sigA (closed square) during M.tb. growth in culture. (b) Using copy number, sigI transcription was analyzed under stress conditions (relative to no stress conditions). The following stress conditions were analyzed: detergent (SDS), disulfide (diamide), oxidative (cumene), acid (pH 4.5), antibiotic (cycloserine, streptomycin, ethambutol, INH, kanamycin), ethanol (EtOH), low iron (No Fe GAST) and heat shock (42°C). . (c) Diagram of the restriction map for Southern blotting of wild-type, ΔsigI and the sigI-complemented (Ico) M.tb. strains. Arrow indicates sigI gene. E: EcoRI restriction site. Hyg: hygromycin resistance gene. (d) Agarose gel of EcoRI-digested genomic DNA for Southern blotting. (e) Southern blot with probe (primers described in Supplementary Table S2). (f) Acid-fast staining of wild-type and ΔsigI mutant M.tb. strains. Scale bar: 5 μm. (g) Growth curves for wild-type (open circle) and ΔsigI (closed square) M.tb. strains. Three biological replicates of all experiments were performed, and error bars represent standard deviation.