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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Nat Commun. 2012 Mar 20;3:753. doi: 10.1038/ncomms1724

Figure 3. SigI transcribes the katG gene.

Figure 3

(a) SigI-containing RNA polymerase holoenzyme was able to direct transcription from the promoter region 379 bp upstream of the katG gene. After transcription by SigI, the gene product was amplified by RT-PCR. The ORF region Rv1186 was used as a negative control for IVT, as SigI does not direct transcription of this gene. (b) Total bacterial lysates from wild-type and ΔsigI strains were utilized as RNA polymerase component in an IVT assay in which the promoter region 33bp upstream of katG was used as the DNA template. (c) Putative SigI binding motif as identified from upstream sequences of the genes identified by microarray to be a part of the SigI regulon. (d) Nucleotide sequence of the katG promoter; the shaded region indicates the deletion. (e) SigI cannot direct transcription from an IVT template in which the putative SigI binding motif has been deleted (from promoter region 33bp upstream of katG, ΔpkatG). Three biological replicates of all experiments were performed.