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. Author manuscript; available in PMC: 2013 May 9.
Published in final edited form as: Eur J Neurosci. 2008 Feb 5;29(4):748–760. doi: 10.1111/j.1460-9568.2008.06606.x

Fig. 4.

Fig. 4

Photomicrographs and quantification of frontal sections showing PRV (black) and CTb (brown) immunoreactivity in putative relay nuclei at 36 h following injection into VTA. (A and C) Low-magnification photomicrographs of frontal sections showing (A) cd-LS and (C) MPON. (B and D) High-magnification photomicrographs of insets in A and C, respectively. Midline is at the right edge of the image in A and center in C. Dorsal is up in all panels. LV, lateral ventricle; CC, corpus callosum; 3 V, third ventricle. (E) Quantification of PRV+ neurons in ipsilateral cd-LS, MPON and nearby nuclei at 36 h following injection into VTA (cv-LS, caudal-ventral lateral septum; PVN, paraventricular nucleus of the hypothalamus; VMH, ventromedial hypothalamus; PVT, paraventricular nucleus of the thalamus). The y-axis indicates the average number of PRV+ neurons per section in the hemisphere ipsilateral to the PRV/CTb injection in VTA. Labeling was higher in cd-LS and MPON than in the four other areas (*P < 0.05 vs. areas in grey bars). Scale bars, 250 μm (A and C), 100 μm (B and D).